Characterization of endoglucanases from the brown rot. Gloeophyllum trabeum is a potent filamentous fungus that rapidly decomposes lignocellulose. When fresh and very young its cap is more or less orange, but as it matures brown colors replace the orange from the center outwards. Files are available under licenses specified on their description page. The transplasma membrane redox system of the brown rot fungus gloeophyllum trabeum was characterized with ferricyanide reduction kinetics. This page was last edited on 14 september 2017, at 19. Characteristics of gloeophyllum trabeum alcohol oxidase, an. Gloeophyllum trabeum, compare this to the several known p2oxs from whiterot fungi, and engineer a cdna for heterologous expression of this enzyme. Request pdf a thermostable gloeophyllum trabeum xylanase with potential for the brewing industry a xylanase gene of glycoside hydrolase family 10, gtxyn10, was cloned from gloeophyllum trabeum. These chelators may function in scavenging transition metals for fungal metabolism and extracellular enzyme production. In this work, gloeophyllum trabeum ku41 was chosen for corn stover pretreatment through screening with 40 strains of woodrot fungi. The endogenous laccase candidate gene gtlcc3 was fused with the promoter of. The enzymes were produced in the heterologous host pichia pastoris. The basidiomycete gloeophyllum trabeum is a representative of the brown rot fungi, which are characterized by a special pattern of wood decay.
Saxs studies of the endoglucanase cel12a from gloeophyllum trabeum show its monomeric structure and reveal the influence of. It has been shown to produce a hydroquinonedriven system for the production of extracellular ros 20, 27, a glucosidase 14, a xylanase 31, and two endoglucanases that were reported not to degrade crystalline cellulose 15, 25. Pretreatment is an essential step in the enzymatic hydrolysis of biomass for bioethanol production. Behavior of the brownrot fungus gloeophyllum trabeum on. Balkenblattling, gloeophyllum trabeum, persoon 1801.
Hammel institute for microbial and biochemical technology, usda forest products laboratory, madison, wi 53705, usa received 28 january 1999 abstract we have identified key components of the extra. Gloeophyllum sepiarium rusty gilled polypore is a wood decay fungus that causes a brown rot. The isolated aox cdna contained an open reading frame of 1,953 bp. Enhancing the catalytic activity of a novel gh5 cellulase. The brownrot basidiomycete gloeophyllum trabeum uses a quinone redox cycle to generate extracellular fenton reagent, a key component of the biodegradative system expressed by this highly destructive wood decay fungus. Hammel 1, institute for microbial and biochemical technology 1 and fiber processing and paper performance research work unit, 2 u. Gloeophyllum sepiarium grows in thin, dark browngreen brackets on dead conifers. Nitrogen deficiency did not statistically affect the ferricyanide reduction rate, which depended on initial ferricyanide concentration and initial mycelial mass. Characterization of a lytic polysaccharide monooxygenase from gloeophyllum trabeum shows a phdependent relationship between catalytic activity and hydrogen peroxide production. All structured data from the file and property namespaces is available under the creative commons cc0 license. A thermostable gloeophyllum trabeum xylanase with potential for the brewing industry. Decomposing the deadwood of conifers across north america, gloeophyllum sepiarium is fairly easily recognized.
The objective of the current work is to find out which characteristics of corn stover pretreated with g. Effects of humic and oxalic acid in wood biodegradation by gloeophyllum trabeum. Characterization of a lytic polysaccharide monooxygenase. To construct a strain better suited for biofuel production from japanese cedar wood, we developed a gene transformation system for g. Characteristics of gloeophyllum trabeum alcohol oxidase, an extracellular source of h2o2 in brown rot decay of wood. Characteristics of gloeophyllum trabeum alcohol oxidase. The isolation and immunolocalization of ironbinding. The dna sequence of cel5b had an open reading frame of 1,077 bp, encoding a protein of 359 amino acid residues with a molecular weight of 47 kda. The structure was elucidated by gas chromatographymass spectrometry through comparison to a. Molecular breeding of lignindegrading brownrot fungus. Gloeophyllum trabeum is a brown rot fungus that has long been considered to belong to the polyporales, but is now recognized as a member of the recently established order gloeophyllales. Pathways for extracellular fenton chemistry in the brown. Behavior of the brownrot fungus gloeophyllum trabeum.
A pyranose 2oxidase gene from the brownrot basidiomycete gloeophyllum trabeum was isolated using homologybased degenerate pcr. The pretreatment of corn stover with gloeophyllum trabeum. Efforts were made to study the effects of various carbon sources on the production of the trailfollowing substance z, z, e3,6,8dodecatrien1ol in the brownrot fungusgloeophyllum trabeum lenzites trabea. Pdf to determine the liquid culture conditions under which the. Annals of the entomological society of america although a brown rot fungus, gloeophyllum trabeum pers.
Insights into lignin degradation and its potential industrial applications. Because of the low molecular mass of the chelatemetal complex below da, and the oxidizing potential of the bound transition metals, certain. Biodegradative mechanism of the brown rot basidiomycete. Control of wood decay by trichoderma gliocladium virens.
Sitedirected mutagenesis based on rational design has been widely used to identify the roles of a specific amino acid residue. The substance is found in a southern subterranean termite,reticulitermes virginicus, and is known to function as the trailfollowing pheromone. Behavior of the brownrot fungus gloeophyllum trabeum on thermallymodified eucalyptus grandis wood. Pdf culture conditions affecting biodegradation components of. Another group of wooddecaying fungi is the brownrot fungi such as gloeophyllum trabeum, laetiporus portentosus, and fomitopsis lilacinogilva, which grow mainly on conifers and represent only 7% of woodrotting.
A novel alcohol oxidase aox has been purified from mycelial pellets of the wooddegrading basidiomycete gloeophyllum trabeum and characterized as a homooctameric nonglycosylated protein with native and subunit molecular masses of 628 and 72. Gene cloning and herologous expression of pyranose 2. Pdf biodegradation inducers to enhance wheat straw. Pretreatment is an essential step in the enzymatic hydrolysis of biomass for bio ethanol production. Author links open overlay panel xiaoyu wang a b 1 huiying luo b 1 wangning yu c rui ma b shuai you b weina liu a lingyu hou a fei zheng a b xiangming xie a bin yao b. The decay pattern in bamboo fibers caused by a brown rot fungus, gloeophyllum trabeum, was. These reactions generate extracellular fenton reagent, which enables g. Characterization of acidic endoglucanase cel12a from. This studys aim was to determine the antioxidant and anticholinesterase activities of gloeophyllum odoratum, gloeophyllum sepiarium, and gloeophyllum trabeum species with phenolic profiles. Characteristics of bifunctional acidic endoglucanase. Brownrot basidiomycetes showed pss depolymerisation of up to 50 % reduction in numberaverage.
Gloeophyllum sepiarium basidiomycota gloeophyllales gloeophyllaceae gloeophyllum. Media in category gloeophyllum trabeum the following 8 files are in this category, out of 8 total. The new dimethoxycatechol 4,5dimethoxy1,2benzenediol dmc and the new dimethoxyhydroquinone 2,5dimethoxy1,4benzenediol dmh were isolated from stationary cultures of the brown rot fungus gloeophyllum trabeum growing on a glucose mineral medium protected from light. Gloeophyllum trabeum and characterized as a homooctameric. The accelerated decay caused by the brownrot fungus g. Pdf characteristics of gloeophyllum trabeum alcohol oxidase, an. Electrical resistance measurements were taken on wood blocks exposed to gloeophyllum trabeum in a standard soil bottle tests awpa e10 as a function of time. The gene structure was determined and compared to that of several pyranose 2oxidases cloned from whiterot fungi. Saxs studies of the endoglucanase cel12a from gloeophyllum. The genus gloeophyllum is characterized by the production of leathery to corky tough, brown, shaggytopped, revivable fruitbodies lacking a stipe and with a lamellate to daedaleoid or poroid fertile hymenial surfaces.
Pdf characteristics of gloeophyllum trabeum alcohol. It has been shown to produce a hydroquinonedriven system for the production of extracellular ros 20, 27, a. Definitions of gloeophyllum trabeum, synonyms, antonyms, derivatives of gloeophyllum trabeum, analogical dictionary of gloeophyllum trabeum english. Potential of woodrotting fungi to attack polystyrene sulfonate and. Murrill, has been shown to be attractive and beneficial to subterranean termites, including coptotermes formosanus shiraki isoptera. In this study, a selection of woodrotting fungi representing different lignocellulose decay types was screened for oxidative biodegradation of the polymer polystyrene sulfonate pss. The effect of a lytic polysaccharide monooxygenase and a. Synthetic polymers often pose environmental hazards due to low biodegradation rates and resulting accumulation. Brown rot fungi have evolved multiple times independently in several lineages. And oxalic acid in wood biodegradation by gloeophyllum trabeum.
Decay, postia placenta, gloeophyllum trabeum, trametes versicolor, douglasfir, western hemlock, southern pine, modulus of elasticity, modulus of. Suppression of growth of a brown rot fungus, gloeophyllum. Egs and egt were isolated from the fungi gloeophyllum sepiarium and gloeophyllum trabeum, respectively, using fractionated ammonium sulfate precipitation, hydrophobic interaction. The purified recombinant gtcel12a exhibited high ph stability and very high specific enzymic activity against. In this work we examined the combined action of two auxiliary proteins from the brown rot fungus gloeophyllum trabeum. The pretreatment of corn stover with gloeophyllum trabeum ku41. Gene cloning and heterologous expression of pyranose 2. Gloeophyllum trabeum balkenblattling aphyllophorales s. The basidiomycete gloeophyllum trabeum ku41 can degrade japanese cedar wood efficiently. Often found on wood in lumberyards, the fruiting body grows for only one year. We prepared degenerate nucleotide primers from several highly conserved regions of whiterot fungi p2ox and ampli.
The endoglucanase cel5b from the filamentous fungus gloeophyllum trabeum was cloned and expressed without a signal peptide, and alanine residue 22 converted to glutamine in pichia pastoris gs115. Its gills are more crowded 24 per mm, its pores are smaller up to 4 per mm along the margin, it usually grows on deciduous wood, and it never has the bright colored growing margin that g. Gloeophyllum trabeum an overview sciencedirect topics. Indepth investigations with the most efficient depolymeriser, a gloeophyllum trabeum strain, pointed at extracellular hydroquinonedriven fenton. Processive endoglucanase active in crystalline cellulose. Rhinotermitidae, no research has been conducted to determine whether the association is mutualistic. The results indicated that the wheat straw pretreatment using g.
Pathways for extracellular fenton chemistry in the brown rot basidiomycete gloeophyllum trabeum kenneth a. Phenolic profiles, antioxidant, and anticholinesterase. Article pdf available in applied and environmental microbiology. Read characterization of endoglucanases from the brown rot fungi gloeophyllum sepiarium and gloeophyllum trabeum, enzyme and microbial technology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. In the present study, a novel cellulase of gh5 from gloeophyllum trabeum cbs 900. Hydrolysis of lignocellulosic biomass depends on the concerted actions of cellulases and accessory proteins. Low molecular weight ironbinding compounds are produced by the brownrot fungus gloeophyllum trabeum. The hitherto uncharacterized quinone reductase that drives this cycle is a potential target for inhibitors of wood decay. Characterization of a transplasma membrane redox system of. Pathways for extracellular fenton chemistry in the brown rot basidiomycete gloeophyllum trabeum. Potential of woodrotting fungi to attack polystyrene. Screening and evaluation of streptomyces species as a.